Increase in Subcellular GSK-3 Clusters in Insulin- and Adrenaline-treated Differentiated Rat Skeletal Muscle Fibres

Authors

  • Katja Fink Faculty of Medicine, University of Ljubljana
  • Mateja Lobe Prebil Faculty of Medicine, University of Ljubljana
  • Nina Vardjan Faculty of Medicine, University of Ljubljana
  • Jorgen Jensen Department of Physical Performance, Norwegian School of Sport Sciences, Postboks 4014 Ullevål stadion, 0806 Oslo, Norway
  • Robert Zorec Faculty of Medicine, University of Ljubljana
  • Marko Kreft Biotechnical Faculty, University of Ljubljana

DOI:

https://doi.org/10.5566/ias.2356

Keywords:

adrenaline, GSK-3 (glycogen synthase kinase 3), insulin, skeletal muscle fibre

Abstract

Glycogen synthase kinase 3 (GSK-3) plays an important role in metabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation. The aim of the present study was to study the effect of insulin and adrenaline on GSK-3 localisation in skeletal muscles. We characterized subcellular localization of (GSK-3) signal protein in fully differentiated muscle fibre by immunofluorescence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of single central optical section of the muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average size were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linearly related to their quantity. We conclude that subcellular GSK-3 in isolated skeletal muscle fibres is localized in clusters and clustering increased after stimulation with insulin and/or adrenaline.

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Published

2020-04-13

How to Cite

Fink, K., Lobe Prebil, M., Vardjan, N., Jensen, J., Zorec, R., & Kreft, M. (2020). Increase in Subcellular GSK-3 Clusters in Insulin- and Adrenaline-treated Differentiated Rat Skeletal Muscle Fibres. Image Analysis and Stereology, 39(1), 25–32. https://doi.org/10.5566/ias.2356

Issue

Vol. 39 No. 1 (2020)

Section

Original Research Paper

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