QUANTIFICATION OF ANGIOGENESIS ON THE RAT AORTIC RING ASSAY

Authors

  • Silvia Blacher
  • Laetitia Devy
  • Agnes Noel
  • Jean-Michel Foidart

DOI:

https://doi.org/10.5566/ias.v22.p43-48

Keywords:

angiogenesis, complexity, fibroblast-like cells, microvessels, quantification

Abstract

Image analysis is used to quantify angiogenesis on the rat aortic ring model. This technique allows to determine: (1) the aortic ring area and factor shape; (2) the number of microvessels, the total number of branching, the maximal microvessel length and the number of microvessels in function of the distance to the aortic ring; (3) the total number of isolated fibroblast-like cells and the number of fibroblast-like cells in function of the distance to the aortic ring. We show that this method is suitable to quantify spontaneous angiogenesis as well as to analyse a complex microvascular network induced by vascular endothelial growth factor (VEGF).

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Published

2011-05-03

How to Cite

Blacher, S., Devy, L., Noel, A., & Foidart, J.-M. (2011). QUANTIFICATION OF ANGIOGENESIS ON THE RAT AORTIC RING ASSAY. Image Analysis and Stereology, 22(1), 43–48. https://doi.org/10.5566/ias.v22.p43-48

Issue

Section

Original Research Paper

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