QUANTIFICATION OF ANGIOGENESIS ON THE RAT AORTIC RING ASSAY

Silvia Blacher, Laetitia Devy, Agnes Noel, Jean-Michel Foidart

Abstract

Image analysis is used to quantify angiogenesis on the rat aortic ring model. This technique allows to determine: (1) the aortic ring area and factor shape; (2) the number of microvessels, the total number of branching, the maximal microvessel length and the number of microvessels in function of the distance to the aortic ring; (3) the total number of isolated fibroblast-like cells and the number of fibroblast-like cells in function of the distance to the aortic ring. We show that this method is suitable to quantify spontaneous angiogenesis as well as to analyse a complex microvascular network induced by vascular endothelial growth factor (VEGF).

Keywords
angiogenesis; complexity; fibroblast-like cells; microvessels; quantification

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DOI: 10.5566/ias.v22.p43-48

Copyright (c) 2014 Image Analysis & Stereology

Image Analysis & Stereology
EISSN 1854-5165 (Electronic version)
ISSN 1580-3139 (Printed version)