STEREOLOGIC ESTIMATION OF KI-67, CASPASE 3 AND GSTP1 POSITIVE CELLS IN PROSTATE LESIONS

Luis Santamaría, Rocío Martín, Vicente Gómez, Ildefonso Ingelmo, Consuelo López, Rafael Revestido

Abstract

Cell proliferation, caspase 3 and pi-form of glutathione S transferase (GSTP1) were evaluated in prostate carcinoma (PCA), proliferative inflammatory atrophy (PIA) and prostate intraepithelial neoplasia (PIN). Forty biopsies were classified as: without morphological lesions (controls: CTR), PIA, PIN and PCA. Ki67, caspase3 and GSTP1 were immunostained. The following estimates were performed: Numerical densities of Ki67+ cells (NVEPKi67), of all epithelial cells (NVEPtotal) and of GSTP1+ cells (NVEPGSTP1); labelling index for Ki67 (LIKi67); volume fraction to caspase 3 positive tissue (VVcaspase 3) and of GSTP1 positive tissue (VVGSTP1). ANOVA was performed to compare the groups. NVEPtotal and NVEPKi67 were increased in PIA. LIKi67 was only increased in PCA. VVcaspase 3 was decreased in PIN and PCA. VVEGSTP1 was decreased in PCA. In our results PIA lacks the characteristics of a premalignant lesion. The result may be explained by the use of unbiased quantitative methods, the inadequate definition of PIA and the scarce inflammation observed in the samples with PIA included in this study.

Keywords
caspase 3; cell proliferation; GSTP1; Ki-67; preneoplasia; prostate; stereology

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DOI: 10.5566/ias.v24.p77-84

Image Analysis & Stereology
EISSN 1854-5165 (Electronic version)
ISSN 1580-3139 (Printed version)