ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY

Barbora Radochová, Jiří Janáček, Kateřina Schwarzerová, Erna Demjénová, Zoltán Tomori, Petr Karen, Lucie Kubínová

Abstract

Image analysis techniques for preprocessing, segmentation and estimation of geometrical characteristics of fiber-like structures from 2-D or 3-D images captured by a confocal microscope are presented. Methods are demonstrated on fiber-like biological structure: endoplasmic reticulum (ER) of tobacco cells. In the presented analysis of 2-D images of ER before and after the treatment of latrunculin B, ER and ER tubules were segmented and the area density of ER as well as the length density of ER tubules in the cell cortical layer were estimated by automatic image analysis algorithms. Images of 3-D arrangement of ER were reconstructed and rendered by various visualization techniques.

Keywords
capillaries; confocal microscopy; endoplasmic reticulum; fibers; image analysis; length density

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DOI: 10.5566/ias.v24.p181-185

Image Analysis & Stereology
EISSN 1854-5165 (Electronic version)
ISSN 1580-3139 (Printed version)